Searching for new vaccine strategies against classical swine fever

A new project granted by the Ministry of Science and Innovation will permit investigating new vaccine strategies against classical swine fever virus (CSFV) and studying the mechanisms of infection and protection. This project is coordinated among the Centre de Recerca en Sanitat Animal (CReSA), Centro de Biología Molecular (CBM SO-CSIC) and Universitat Pompeu Fabra (UPF).

Classical swine fever virus (CSFV) is the causative agent of one of the most devastating porcine haemorrhagic viral diseases, classical swine fever (CSF). Efficient live attenuated vaccines against CSFV exist and are routinely used only in endemic countries. The ability of these vaccines to replicate in the host, even at very low rates, makes it extremely difficult to distinguish vaccinated from infected animals, favouring a restricted policy regarding vaccination against CSFV in non-endemic countries. There is a clear need for efficient and safer marker vaccines to assist in the control of future CSF outbreakss.

The project entitled “New vaccine strategies against CSFV. Study of the mechanisms of viral immunopathogenicity” will permit studying different mechanisms of protection against CSFV for developing new vaccination strategies and rapid infection diagnostic methods.

The main purposes of this project are:

  • Development of new vaccine strategies against CSFV, based on the use of dendrimeric peptides combining B-cell and T-cell epitopes and in DNA vaccines.
  • Characterization of the immunological mechanisms associated with protection against disease.
  • Study of the mechanisms of viral pathogenicity in pigs infected with strains of different virulence.
  • Development of new strategies based on immunological and molecular techniques for the rapid diagnosis of CSFV.
  • Studies of viral evolution in strains isolated from pigs during 20 years of circulation of the virus in the field.

The CReSA’s team involved in the development of the project is made of: Dr. Mariano Domingo, Dr. Llilianne Ganges, Dr. Lorenzo Fraile, Dr. Francesc Xavier Abad, Iván Galindo and Joan Tarradas.

To contact with the investigator in charge for the project at CReSA:

Dr. Llilianne Ganges Espinosa
Viral Diseases Unit
Edifici CReSA. Campus UAB
08193 Bellaterra (Barcelona) España
Telephone no.: +34 93.581.46.20
Fax: +34 93 581 44 90

To know about Dr. Ganges publications on CSFV (Pubmed):

Classical swine fever virus E2 glycoprotein antigen produced in adenovirally transduced PK-15 cells confers complete protection in pigs upon viral challenge.
Sánchez O, Barrera M, Rodríguez MP, Frías MT, Figueroa NE, Naranjo P, Montesino R, Farnos O, Castell S, Venereo A, Ganges L, Borroto C, Toledo JR.
Vaccine. 2008 Feb 13;26(7):988-97. Epub 2007 Nov 26.
Highly protective E2-CSFV vaccine candidate produced in the mammary gland of adenoviral transduced goats.
Toledo JR, Sánchez O, Montesino R, Farnos O, Rodríguez MP, Alfonso P, Oramas N, Rodríguez E, Santana E, Vega E, Ganges L, Frias MT, Cremata J, Barrera M.
J Biotechnol. 2008 Feb 1;133(3):370-6. Epub 2007 Oct 5.
Recent advances in the development of recombinant vaccines against classical swine fever virus: cellular responses also play a role in protection.
Ganges L, Núñez JI, Sobrino F, Borrego B, Fernández-Borges N, Frías-Lepoureau MT, Rodríguez F.
Vet J. 2008 Aug;177(2):169-77. Epub 2007 Sep 4. Review.
A DNA vaccine expressing the E2 protein of classical swine fever virus elicits T cell responses that can prime for rapid antibody production and confer total protection upon viral challenge.
Ganges L, Barrera M, Núñez JI, Blanco I, Frias MT, Rodríguez F, Sobrino F.
Vaccine. 2005 May 25;23(28):3741-52.
Origin and evolution of viruses causing classical swine fever in Cuba.
de Arce HD, Ganges L, Barrera M, Naranjo D, Sobrino F, Frías MT, Núñez JI.
Virus Res. 2005 Sep;112(1-2):123-31.
Molecular epidemiology of classical swine fever in Cuba.
Díaz de Arce H, Núñez JI, Ganges L, Barreras M, Teresa Frías M, Sobrino F.
Virus Res. 1999 Oct;64(1):61-7.
An RT-PCR assay for the specific detection of classical swine fever virus in clinical samples.
Díaz de Arce H, Nuñez JI, Ganges L, Barreras M, Frías MT, Sobrino F.
Vet Res. 1998 Sep-Oct;29(5):431-40.

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